Hi it was a very informative ppt. However I have a one doubt related to my project on liposome.
I am developing ethosome (instead of water, water- ethanol system as hydration medium) and liposome of my drug. But when I centrifuge at 13000 rpm for 30 mins twice (as suggested in various research paper which used the same composition), instead of getting pellet at the bottom, I get fluffy mass. And when I want to collect supernatant, some of the fluffy mass escape in the supernatant.
If I try just to collect carefully the supernatant, I am left with fluffy mass at the bottom with 1-2 ml hydration medium...which is then very difficult to dry or lyophilize.
I would appreciate if you knows the reason of getting fluffy mass or if its normal to get in case of liposome and ethosomes.
Kind Regards
